Center for Integrative Biomedical Computing

SCI Publications


B.M. Isaacson, J.G. Stinstra, R.S. MacLeod, J.B. Webster, J.P. Beck, R.D. Bloebaum. “Bioelectric Analyses of an Osseointegrated Intelligent Implant Design System for Amputees,” In JoVE, Vol. 29, 2009.

M. Kamali, L.J. Day, D.H. Brooks, X. Zhou, D.M. O'Malley. “Automated identification of neurons in 3D confocal datasets from zebrafish brainstem,” In Journal of Microscopy, Vol. 233, No. 1, pp. 114--131. January, 2009.
DOI: 10.1111/j.1365-2818.2008.03102.x
PubMed ID: 19196418
PubMed Central ID: PMC2798854


Many kinds of neuroscience data are being acquired regarding the dynamic behaviour and phenotypic diversity of nerve cells. But as the size, complexity and numbers of 3D neuroanatomical datasets grow ever larger, the need for automated detection and analysis of individual neurons takes on greater importance. We describe here a method that detects and identifies neurons within confocal image stacks acquired from the zebrafish brainstem. The first step is to create a template that incorporates the location of all known neurons within a population - in this case the population of reticulospinal cells. Once created, the template is used in conjunction with a sequence of algorithms to determine the 3D location and identity of all fluorescent neurons in each confocal dataset. After an image registration step, neurons are segmented within the confocal image stack and subsequently localized to specific locations within the brainstem template - in many instances identifying neurons as specific, individual reticulospinal cells. This image-processing sequence is fully automated except for the initial selection of three registration points on a maximum projection image. In analysing confocal image stacks that ranged considerably in image quality, we found that this method correctly identified on average approximately 80% of the neurons (if we assume that manual detection by experts constitutes 'ground truth'). Because this identification can be generated approximately 100 times faster than manual identification, it offers a considerable time savings for the investigation of zebrafish reticulospinal neurons. In addition to its cell identification function, this protocol might also be integrated with stereological techniques to enhance quantification of neurons in larger databases. Our focus has been on zebrafish brainstem systems, but the methods described should be applicable to diverse neural architectures including retina, hippocampus and cerebral cortex.

J. Krüger, T. Fogal. “Focus and Context - Visualization without the Complexity,” In Proceedings of the World Congress on Medical Physics and Biomedical Engineering, September 7 - 12, 2009, Munich, Germany, IFMBE Proceedings, Vol. 25/13, Springer Berlin Heidelberg, pp. 44--48. 2009.

S. Lew, C.H. Wolters, T. Dierkes, C. Röer, R.S. MacLeod. “Accuracy and run-time comparison for different potential approaches and iterative solvers in finite element method based EEG source analysis,” In Applied Numerical Mathematics, Vol. 59, pp. 1970--1988. 2009.

S. Lew, C.H. Wolters, A. Anwander, S. Makeig, R.S. MacLeod. “Improved EEG Source Analysis Using Low-Resolution Conductivity Estimation in a Four-Compartment Finite Element Head Model,” In Human Brain Mapping, Vol. 30, pp. 2862--2878. 2009.

R.S. MacLeod, J.G. Stinstra, S. Lew, R.T. Whitaker, D.J. Swenson, M.J. Cole, J. Krüger, D.H. Brooks, C.R. Johnson. “Subject-specific, multiscale simulation of electrophysiology: a software pipeline for image-based models and application examples,” In Philosophical Transactions of The Royal Society A, Mathematical, Physical & Engineering Sciences, Vol. 367, No. 1896, pp. 2293--2310. 2009.

H.G. Martinez, S.I. Prajapati, C.A. Estrada, F. Jimenez, M.P. Quinones, I. Wu, A. Bahadur, A. Sanderson, C.R. Johnson, M. Shim, C. Keller, S.S. Ahuja. “Microscopic Computed Tomography Based Virtual Histology for Visualization and Morphometry of Atherosclerosis in Diabetic Apolipoprotein E Mutant Mice,” In Circulation: Journal of the American Heart Association, Vol. 120, No. 9, pp. 821--822. 2009.

H. Martinez, S. Prajapati, C. Estrada, F. Jimenez, I. Wu, A. Bahadur, A. Sanderson, C.R. Johnson, M. Shim, C. Keller, S. Ahuja. “Microscopic Computed Tomography–Based Virtual Histology for Visualization and Morphometry of Atherosclerosis in Diabetic Apolipoprotein E Mutant Mice,” In Circulation, Vol. 120, No. 821--822, 2009.

M. Milanic, V. Jazbinsek, D.F. Wang, J. Sinstra, R.S. Macleod, D.H. Brooks, R. Hren. “Evaluation of Approaches of Solving Electrocardiographic Imaging Problem,” In Proceeding of Computers in Cardiology 2010, Park City, Utah, September, 2009.

R.S. Oakes, T.J. Badger, E.G. Kholmovski, N. Akoum, N.S. Burgon, E.N. Fish, J.J. Blauer, S.N. Rao, E.V. DiBella, N.M. Segerson, M. Daccarett, J. Windfelder, C.J. McGann, D.L. Parker, R.S. MacLeod, N.F. Marrouche. “Detection and quantification of left atrial structural remodeling with delayed-enhancement magnetic resonance imaging in patients with atrial fibrillation,” In Circulation, Vol. 119, No. 13, pp. 1758--1767. 2009.

I. Oguz, M. Niethammer, J. Cates, R.T. Whitaker, P.T. Fletcher, C. Vachet, M. Styner. “Cortical Correspondence with Probabilistic Fiber Connectivity,” In Information Processing in Medical Imaging (IPMI), Lecture Notes in Computer Science (LCNS), Vol. 5636, pp. 651--663. 2009.
DOI: 10.1007/978-3-642-02498-6_54

S.G. Parker, K. Damevski, A. Khan, A. Swaminathan, C.R. Johnson. “The SCIJump Framework for Parallel and Distributed Scientific Computing,” In Advanced Computational Infrastructures for Parallel and Distributed Adaptive Applications, Edited by Manish Parashar and Xiaolin Li and Sumir Chandra, Wiley-Blackwell, pp. 149--170. 2009.
DOI: 10.1002/9780470558027.ch9

A.R. Sanderson, M.D. Meyer, R.M. Kirby, C.R. Johnson. “A Framework for Exploring Numerical Solutions of Advection Reaction Diffusion Equations using a GPU Based Approach,” In Journal of Computing and Visualization in Science, Vol. 12, pp. 155--170. 2009.
DOI: 10.1007/s00791-008-0086-0

N.M. Segerson, M. Daccarett, T.J. Badger, A. Shabaan, N. Akoum, E.N. Fish, S. Rao, N.S. Burgon, Y. Adjei-Poku, E.G. Kholmovski, S. Vijayakumar, E.V.R. Dibella, R.S. Macleod, N.F. Marrouche. “Magnetic Resonance Imaging-Confirmed Ablative Debulking of the Left Atrial Posterior Wall and Septum for Treatment of Persistent Atrial Fibrillation: Rationale and Initial Experience,” In Journal of Cardiovascular Electrophysiology, Vol. 21, No. 2, pp. 126--132. 2009.

J.F. Shepherd, C.R. Johnson. “Hexahedral Mesh Generation for Biomedical Models in SCIRun,” In Engineering with Computers, Vol. 25, No. 1, pp. 97--114. 2009.

R. Tao, P.T. Fletcher, R.T. Whitaker. “A Variational Image-Based Approach to the Correction of Susceptibility Artifacts in the Alignment of Diffusion Weighted and Structural MRI,” In Lecture Notes in Computer Science, Springer, pp. 664--675. 2009.
DOI: 10.1007/978-3-642-02498-6_55
PubMed ID: 19694302

J.D. Tate, J.G. Stinstra, T. Pilcher, and R.S. MacLeod. “Measuring Implantable Cardioverter Defibrillators (ICDs) During Implantation Surgery: Verification of a Simulation,” In Computers in Cardiology, pp. 473--476. 2009.
ISSN: 0276-6547


Implantable cardioverter defibrillators (ICDs) are increasing used in abnormal configurations. We have developed a patient specific forward simulation model to predict efficacy of the defibrillation shock. Our goal was to develop a method of measuring the ICD surface potentials as the devices are tested during implantation surgery to use as verification of the simulation. A lead selection algorithm was used to develop a surface potential mapping system with 32 recording sites that do not interfere with implantation surgery. ICD discharge recordings were compared at similar locations to corresponding patient models. The reconstructed simulated surface potentials showed

D.F. Wang, R.M. Kirby, C.R. Johnson. “Finite Element Discretization Strategies for the Inverse Electrocardiographic (ECG) Problem,” In Proceedings of the 11th World Congress on Medical Physics and Biomedical Engineering, Munich, Germany, Vol. 25/2, pp. 729-732. September, 2009.

D.F. Wang, R.M. Kirby, C.R. Johnson. “Finite Element Refinements for Inverse Electrocardiography: Hybrid-Shaped Elements, High-Order Element Truncation and Variational Gradient Operator,” In Proceeding of Computers in Cardiology 2009, Park City, September, 2009.


J. Cates, P.T. Fletcher, Z. Warnock, R.T. Whitaker. “A Shape Analysis Framework for Small Animal Phenotyping with Application to Mice with a Targeted Disruption of Hoxd11,” In Proceedings of the 5th IEEE International Symposium on Biomedical Imaging (ISBI '08), pp. 512--516. 2008.
DOI: 10.1109/ISBI.2008.4541045