Center for Integrative Biomedical Computing

SCI Publications


C. Jones, M. Seyedhosseini, M. Ellisman, T. Tasdizen. “Neuron Segmentation in Electron Microscopy Images Using Partial Differential Equations,” In Proceedings of 2013 IEEE 10th International Symposium on Biomedical Imaging (ISBI), pp. 1457--1460. April, 2013.
DOI: 10.1109/ISBI.2013.6556809


In connectomics, neuroscientists seek to identify the synaptic connections between neurons. Segmentation of cell membranes using supervised learning algorithms on electron microscopy images of brain tissue is often done to assist in this effort. Here we present a partial differential equation with a novel growth term to improve the results of a supervised learning algorithm. We also introduce a new method for representing the resulting image that allows for a more dynamic thresholding to further improve the result. Using these two processes we are able to close small to medium sized gaps in the cell membrane detection and improve the Rand error by as much as 9\% over the initial supervised segmentation.

K.B. Jones, M. Datar, S. Ravichandran, H. Jin, E. Jurrus, R.T. Whitaker, M.R. Capecchi. “Toward an Understanding of the Short Bone Phenotype Associated with Multiple Osteochondromas,” In Journal of Orthopaedic Research, Vol. 31, No. 4, pp. 651--657. 2013.
DOI: 10.1002/jor.22280
PubMed ID: 23192691
PubMed Central ID: PMC3683979


Individuals with multiple osteochondromas (MO) demonstrate shortened long bones. Ext1 or Ext2 haploinsufficiency cannot recapitulate the phenotype in mice. Loss of heterozygosity for Ext1 may induce shortening by steal of longitudinal growth into osteochondromas or by a general derangement of physeal signaling. We induced osteochondromagenesis at different time points during skeletal growth in a mouse genetic model, then analyzed femora and tibiae at 12 weeks using micro-CT and a point-distribution-based shape analysis. Bone lengths and volumes were compared. Metaphyseal volume deviations from normal, as a measure of phenotypic widening, were tested for correlation with length deviations. Mice with osteochondromas had shorter femora and tibiae than controls, more consistently when osteochondromagenesis was induced earlier during skeletal growth. Volumetric metaphyseal widening did not correlate with longitudinal shortening, although some of the most severe shortening was in bones with abundant osteochondromas. Loss of heterozygosity for Ext1 was sufficient to drive bone shortening in a mouse model of MO, but shortening did not correlate with osteochondroma volumetric growth. While a steal phenomenon seems apparent in individual cases, some other mechanism must also be capable of contributing to the short bone phenotype, independent of osteochondroma formation. Clones of chondrocytes lacking functional heparan sulfate must blunt physeal signaling generally, rather than stealing growth potential focally. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 651-657, 2013.


J.R. Anderson, B.C. Grimm, S. Mohammed, B.W. Jones, T. Tasdizen, J. Spaltenstein, P. Koshevoy, R.T. Whitaker, R.E. Marc. “The Viking Viewer: Scalable Multiuser Annotation and Summarization of Large Volume Datasets,” In Journal of Microscopy, Vol. 241, No. 1, pp. 13--28. 2010.
DOI: 10.1111/j.1365-2818.2010.03402.x


S.X. Vasquez, M.S. Hansen, A.N. Bahadur, M.F. Hockin, G.L. Kindlmann, L. Nevell, I.Q. Wu, D.J. Grunwald, D.M. Weinstein, G.M. Jones, C.R. Johnson, J.L. Vandeberg, M.R. Capecchi, C. Keller. “Optimization of Volumetric Computed Tomography for Skeletal Analysis of Model Genetic Organisms,” In The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, Vol. 291, pp. 475--487. 2008.
PubMed ID: 18286615


S. Browd, L.J. Healy, G. Dobie, J.T. Johnson III, G.M. Jones, L.F. Rodriguez, D.L. Brockmeyer. “Morphometric and Qualitative Analysis of Congenital Occipitocervical Instability in Children: Implications for Down Syndrome Patients,” In Journal of Neurosurgery: Pediatrics, Vol. 105, No. 1 , Journal of Neurosurgery Publishing Group, pp. 50--54. July, 2006.
DOI: 10.3171/ped.2006.105.1.50

J.T. Johnson III, M.S. Hansen, I. Wu, L.J. Healy, C.R. Johnson, G.M. Jones, M.R. Capecchi, C. Keller. “Virtual Histology of Transgenic Mouse Embryos for High-Throughput Phenotyping,” In PLoS Genetics, Vol. 2, No. 1, pp. 471--477. April, 2006.

J.T. Johnson III, M.S. Hansen, I.Q Wu, L.J. Healy, C.R. Johnson, G.M. Jones, M.R. Capecchi, C.Keller. “Virtual Histology of Transgenic Mouse Embryos for High-Throughput Phenotyping,” In Proceedings of the Teratology 46th Annual Meeting, Tucson, AZ, 2006.


P. Hawkes, G. Kindlmann, D. Weinstein, G.M. Jones, C. Keller. “Practical Vessel Imaging by Computed Tomography in Live Transgenic Mouse Models for Human Tumors,” In Proceedings of 2005 SMI Conference, 4th Annual Meeting of the Society for Molecular Imaging, Cologne, Germany, 2005.

G.L. Kindlmann, D.M. Weinstein, G.M. Jones, C.R. Johnson, M.R. Capecchi, C. Keller. “Practical Vessel Imaging by Computed Tomography in Live Transgenic Mouse Models for Human Tumors,” In Journal of Molecular Imaging, Vol. 4, No. 4, pp. 417--424. 2005.


J. Cates, R.T. Whitaker, G.M. Jones. “Case Study: An Evaluation of User-Assisted Hierarchical Watershed Segmentation,” No. UUCS-04-006, University of Utah School of Computing, 2004.